Publications (selected)

2021

• Fischer*, Rieblinger* (Co-first author), Hein R, Sfriso R, Zuber J, Schäffler A, Klinger B, Wei L, Flisikowski K, Kurome M, Zakhartchenko V, Kessler B, Wolf E, Rieben R, Schwinzer R, Kind A, Schnieke A. Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2. Xenotransplantation,  27(1):e12560.

• Hinrichs A, Riedel EO, Klymiuk N, Blutke A, Kemter E, Längin M, Dahlhoff M, Keßler B, Kurome M, Zakhartchenko V, Jemiller EM, Ayares D, Bidlingmaier M, Flenkenthaler F, Hrabĕ de Angelis M, Arnold GJ, Reichart B, Fröhlich T, Wolf E. Growth hormone receptor knockout to reduce the size of donor pigs for preclinical xenotransplantation studies. Xenotransplantation. 2021 Mar;28(2):e12664. doi: 10.1111/xen.12664. Epub 2020 Nov 25. PMID: 33241624

• Kemter E, Citro A, Wolf-van Buerck L, Qiu Y, Böttcher A, Policardi M, Pellegrini S, Valla L, Alunni-Fabbroni M, Kobolak J, Kessler B, Kurome M, Zakhartchenko V, Dinnyes A, Cyran C, Lickert H, Piemonti L, Seissler J, Wolf E. Transgenic pigs expressing near infrared fluorescent protein—A novel tool for noninvasive imaging of islet xenotransplants. Xenotransplantation, 21 December; doi.org/10.1111/xen.12719

2020

• Reichart B, Längin M, Radan J, Mokelke M, Buttgereit I, Ying J, Fresch AK, Mayr T, Issl L, Buchholz S, Michel S, Ellgass R, Mihalj M, Egerer S, Baehr A, Kessler B, Kemter E, Kurome M, Zakhartchenko V, Steen S, Sjöberg T, Paskevicius A, Krüger L, Fiebig U, Denner J, Godehardt AW, Tönjes RR, Milusev A, Rieben R, Sfriso R, Walz C, Kirchner T, Ayares D, Lampe K, Schönmann U, Hagl C, Wolf E, Klymiuk N, Abicht JM, Brenner P. Pig-to-non-human primate heart transplantation: The final step toward clinical xenotransplantation? J Heart Lung Transplant. Aug;39(8):751-757.

2018

• Hinrichs A, Kessler B, Kurome M, Blutke A, Kemter E, Bernau M, Scholz AM, Rathkolb B, Renner S, Bultmann S, Leonhardt H, de Angelis MH, Nagashima H, Hoeflich A, Blum WF, Bidlingmaier M, Wanke R, Dahlhoff M, Wolf E. Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver. Mol Metab. May;11:113-128. doi: 10.1016/j.molmet.2018.03.006. Epub 2018 Mar 15.

• Egerer S, Fiebig U, Kessler B, Zakhartchenko V, Kurome M, Reichart B, Kupatt C, Klymiuk N, Wolf E, Denner J, Bähr A. Early weaning completely eliminates porcine cytomegalovirus from a newly established pig donor facility for xenotransplantation. Xenotransplantation Jul. 25(4):e12449
  
  
• Kurome M, Baehr A, Simmet K, Jemiller EM, Egerer S, Dahlhoff M, Zakhartchenko V, Nagashima H, Klymiuk N, Kessler B, Wolf E. Targeting αGal epitopes for multi-species embryo immunosurgery. Reprod Fertil Dev. 2018 Nov 2. doi: 10.1071/RD18120.
  
  
• Längin M, Mayr T, Reichart B, Michel S, Buchholz S, Guethoff S, Dashkevich A, Baehr A, Egerer S, Bauer A, Mihalj M, Panelli A, Issl L, Ying J, Fresch AK, Buttgereit I, Mokelke M, Radan J, Werner F, Lutzmann I, Steen S, Sjöberg T, Paskevicius A, Qiuming L, Sfriso R, Rieben R, Dahlhoff M, Kessler B, Kemter E, Kurome M, Zakhartchenko V, Klett K, Hinkel R, Kupatt C, Falkenau A, Reu S, Ellgass R, Herzog R, Binder U, Wich G, Skerra A, Ayares D, Kind A, Schönmann U, Kaup FJ, Hagl C, Wolf E, Klymiuk N, Brenner P, Abicht J. Consistent success in life-supporting porcine cardiac xenotransplantation. Nature 5. Dez.;564, pages430–4
  
  
• Rieblinger B, Fischer K, Kind A, Saller BS, Baars W, Schuster M, Wolf-van Buerck L, Schäffler A, Flisikowska T, Kurome M, Zakhartchenko V, Kessler B, Flisikowski K, Wolf E, Seissler J, Schwinzer R, Schnieke A. Strong xenoprotective function by single-copy transgenes placed sequentially at a permissive locus. Xenotransplantation Jan 23. doi: 10.1111/xen.12382.

2017

• Kemter E, Cohrs CM, Schäfer M, Schuster M, Steinmeyer K, Wolf-van Buerck L, Wolf A, Wuensch A, Kurome M, Kessler B, Zakhartchenko V, Loehn M, Ivashchenko Y, Seissler J, Schulte AM, Speier S, Wolf E. INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters. Diabetologia 60, 1152-1156.
  

2016

• Bähr A, Käser T, Kemter E, Gerner W, Kurome M, Baars W, Herbach N, Witter K, Wünsch A, Talker SC, Kessler B, Nagashima H, Saalmüller A, Schwinzer R, Wolf E, Klymiuk N. Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs. PLoS One. 2016 May 13;11(5):e0155676. doi: 10.1371/journal.pone.0155676.
  
  
• Fischer K, Kraner-Scheiber S, Petersen B, Rieblinger B et al. Efficient production of multi-modified pigs for xenotransplantation by cobineering, gene stacking and gene editing. Nature Scientific Reports 2016; doi: 10.1038/srep29081

2015

• Kurome M, Kessler B, Wuensch A, Nagashima H, Wolf E. Nuclear transfer and transgenesis in the pig. Methods Mol Biol;1222:37-59.

 2014

• Li S, Flisikowska T, Kurome M, Zakhartchenko V, Kessler B, Saur D, Kind A, Wolf E, Flisikowski K, Schnieke A. Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus. PLoS One; 9(7):e102455.
  
  
• Klymiuk N, Fezert P, Wünsch A, Kurome M, Kessler B, Wolf E. Homologous recombination contributes to the repair of zinc-finger-nuclease induced double strand breaks in pig primary cells and facilitates recombination with exogenous DNA. J Biotechnol;177:74-81.
  
  
• Wuensch A, Baehr A, Bongoni AK, Kemter E, Blutke A, Baars W, Haertle S, Zakhartchenko V, Kurome M, Kessler B, Faber C, Abicht JM, Reichart B, Wanke R, Schwinzer R, Nagashima H, Rieben R, Ayares D, Wolf E, Klymiuk N. Regulatory sequences of the porcine THBD gene facilitate endothelial-specific expression of bioactive human thrombomodulin in single- and multitransgenic pigs. Transplantation. 97(2):138-47.

 2013

• Klymiuk N, Blutke A, Graf A, Krause S, Burkhardt K, Wuensch A, Krebs S, Kessler B, Zakhartchenko V, Kurome M, Kemter E, Nagashima H, Schoser B, Herbach N, Blum H, Wanke R, Aartsma-Rus A, Thirion C, Lochmüller H, Walter MC, Wolf E. Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle. Hum Mol Genet; 22(21):4368-82.
  
  
• Kurome M, Geistlinger L, Kessler B, Zakhartchenko V, Klymiuk N, Wuensch A, Richter A, Baehr A, Kraehe K, Burkhardt K, Flisikowski K, Flisikowska T, Merkl C, Landmann M, Durkovic M, Tschukes A, Kraner S, Schindelhauer D, Petri T, Kind A, Nagashima H, Schnieke A, Zimmer R, Wolf E. Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set.  BMC Biotechnol;13:43.
  
  
• Renner S, Braun-Reichhart C, Blutke A, Herbach N, Emrich D, Streckel E, Wünsch A, Kessler B, Kurome M, Bähr A, Klymiuk N, Krebs S, Puk O, Nagashima H, Graw J, Blum H, Wanke R, Wolf E. Permanent neonatal diabetes in INS(C94Y) transgenic pigs. Diabetes;62(5):1505-11.

2012

• Richter A, Kurome M, Kessler B, Zakhartchenko V, Klymiuk N, Nagashima H, Wolf E, Wuensch A. Inactivation and inducible oncogenic mutation of p53 in gene targeted pigs. BMC Biotechnol;12:84.
  
  
• Leuchs S, Saalfrank A, Merkl C, Flisikowska T, Edlinger M, Durkovic M, Rezaei N, Kurome M, Zakhartchenko V, Kessler B, Flisikowski K, Kind A, Wolf E, Schnieke A. Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig. PLoS One;7(10):e43323.
  
  
• Flisikowska T, Merkl C, Landmann M, Eser S, Rezaei N, Cui X, Kurome M, Zakhartchenko V, Kessler B, Wieland H, Rottmann O, Schmid RM, Schneider G, Kind A, Wolf E, Saur D, Schnieke A. A porcine model of familial adenomatous polyposis. Gastroenterology;143(5):1173-5.e1-7.
  
  
• Klymiuk N, van Buerck L, Bähr A, Offers M, Kessler B, Wuensch A, Kurome M, Thormann M, Lochner K, Nagashima H, Herbach N, Wanke R, Seissler J, Wolf E. Xenografted islet cell clusters from INSLEA29Y transgenic pigs rescue diabetes and prevent immune rejection in humanized mice. Diabetes;61(6):1527-32.
  
  
• Klymiuk N, Mundhenk L, Kraehe K, Wuensch A, Plog S, Emrich D, Langenmayer MC, Stehr M, Holzinger A, Kröner C, Richter A, Kessler B, Kurome M, Eddicks M, Nagashima H, Heinritzi K, Gruber AD, Wolf E. Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis. J Mol Med (Berl;90 (5):597-608.
  
  
• Klymiuk N, Böcker W, Schönitzer V, Bähr A, Radic T, Fröhlich T, Wünsch A, Keßler B, Kurome M, Schilling E, Herbach N, Wanke R, Nagashima H, Mutschler W, Arnold GJ, Schwinzer R, Schieker M, Wolf E. First inducible transgene expression in porcine large animal models. FASEB J;26(3):1086-99.
  
  
• Watanabe M, Kurome M, Matsunari H, Nakano K, Umeyema K, Shiota A, Nakauchi H, Nagashima H. The creation of transgenic pigs expressing human proteins using BAC-derived, full-length genes and intracytoplasmic sperm injection-mediated gene transfer. Transgenic Res;21(3):605-18.

2011

• Nakano K, Matsunari H, Nakayama N, Ogawa B, Kurome M, Takahashi M, Matsumoto M, Murakami H, Kaji Y, Nagashima H. Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage. J Reprod Dev;57(2):312-6.

 2010

• Aigner B, Renner S, Kessler B, Klymiuk N, Kurome M, Wünsch A, Wolf E. Transgenic pigs as models for translational biomedical research. J Mol Med (Berl);88(7):653-64.

2009

• Umeyama K, Watanabe M, Saito H, Kurome M, Tohi S, Matsunari H, Miki K, Nagashima H. Dominant-negative mutant hepatocyte nuclear factor 1alpha induces diabetes in transgenic-cloned pigs. Transgenic Res;18(5):697-706.
  
  
• Tomii R, Kurome M, Wako N, Ochiai T, Matsunari H, Kano K, Nagashima H. Production of cloned pigs by nuclear transfer of preadipocytes following cell cycle synchronization by differentiation induction. J Reprod Dev;55(2):121-7.

2008

• Matsunari H, Onodera M, Tada N, Mochizuki H, Karasawa S, Haruyama E, Nakayama N, Saito H, Ueno S, Kurome M, Miyawaki A, Nagashima H. Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange. Cloning Stem Cells;10(3):313-23.
  
  
• Kurome M, Hisatomi H, Matsumoto S, Tomii R, Ueno S, Hiruma K, Saito H, Nakamura K, Okumura K, Matsumoto M, Kaji Y, Endo F, Nagashima H. Production efficiency and telomere length of the cloned pigs following serial somatic cell nuclear transfer.  J Reprod Dev;54(4):254-8.
  
  
• Kurome M, Tomii R, Ueno S, Hiruma K, Matsumoto S, Okumura K, Nakamura K, Matsumoto M, Kaji Y, Endo F, Nagashima H. Production of cloned pigs from salivary gland-derived progenitor cells. Cloning Stem Cells. ;10(2):277-86.
  
  
• Kurome M, Ishikawa T, Tomii R, Ueno S, Shimada A, Yazawa H, Nagashima H. Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer.  J Reprod Dev;54(3):156-63.
  
  
• Fujimura T, Murakami H, Kurome M, Takahagi Y, Shigehisa T, Nagashima H. Effects of recloning on the efficiency of production of alpha 1,3-galactosyltransferase knockout pigs. J Reprod Dev;54(1):58-62.
  
  
• Kurome M, Tomii R, Ueno S, Hiruma K, Matsumoto S, Okumura K, Nakamura K, Matsumoto M, Kaji Y, Endo F, Nagashima H. Production of cloned pigs from salivary gland-derived progenitor cells. Cloning Stem Cells 10, 277-86.

2007

• Kurome M, Saito H, Tomii R, Ueno S, Hiruma K, Nagashima H. Effects of sperm pretreatment on efficiency of ICSI-mediated gene transfer in pigs.  J Reprod Dev;53(6):1217-26.
  
  
• Ueno S, Kurome M, Tomii R, Hiruma K, Saitoh H, Nagashima H. Association between embryonic loss and damage to the zona pellucida by invasive micromanipulation during oviductal transfer of early-stage embryos in pigs. J Reprod Dev;53(5):1113-8.
  
  
• Nagashima H, Hiruma K, Saito H, Tomii R, Ueno S, Nakayama N, Matsunari H, Kurome M. Biol Reprod. Production of live piglets following cryopreservation of embryos derived from in vitro-matured oocytes;76(5):900-5.
  
  
• Kurome M, Ueda H, Tomii R, Naruse K, Nagashima H. Transgenic Res. Production of transgenic-clone pigs by the combination of ICSI-mediated gene transfer with somatic cell nuclear transfer; 15(2):229-40.

2006

• Kurome M, Ueda H, Tomii R, Naruse K, Nagashima H. Production of transgenic-clone pigs by the combination of ICSI-mediated gene transfer with somatic cell nuclear transfer. Transgenic Res 15, 229-40.

2003

• Kurome M, Fujimura T, Murakami H, Takahagi Y, Wako N, Ochiai T, Miyazaki K, Nagashima H. Comparison of electro-fusion and intracytoplasmic nuclear injection methods in pig cloning. Cloning Stem Cells 5, 367-78.