Publications (selected)

2023

• Fischer N, Gulich B, Keßler B, Längin M, Fishman JA, Wolf E, Boller K, Tönjes RR, Godehardt AW. PCR and peptide based PCMV detection in pig - development and application of a combined testing procedure differentiating newly from latent infected pigs. Xenotransplantation. 30:e12803. doi: 10.1111/xen.12803. Online ahead of print.

2021

• Fischer*, Rieblinger* (Co-first author), Hein R, Sfriso R, Zuber J, Schäffler A, Klinger B, Wei L, Flisikowski K, Kurome M, Zakhartchenko V, Kessler B, Wolf E, Rieben R, Schwinzer R, Kind A, Schnieke A. Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2. Xenotransplantation,  27(1):e12560.
  
  
• Hinrichs A, Riedel EO, Klymiuk N, Blutke A, Kemter E, Längin M, Dahlhoff M, Keßler B, Kurome M, Zakhartchenko V, Jemiller EM, Ayares D, Bidlingmaier M, Flenkenthaler F, Hrabĕ de Angelis M, Arnold GJ, Reichart B, Fröhlich T, Wolf E. Growth hormone receptor knockout to reduce the size of donor pigs for preclinical xenotransplantation studies. Xenotransplantation. 2021 Mar;28(2):e12664. doi: 10.1111/xen.12664. Epub 2020 Nov 25. PMID: 33241624

• Kemter E, Citro A, Wolf-van Buerck L, Qiu Y, Böttcher A, Policardi M, Pellegrini S, Valla L, Alunni-Fabbroni M, Kobolak J, Kessler B, Kurome M, Zakhartchenko V, Dinnyes A, Cyran C, Lickert H, Piemonti L, Seissler J, Wolf E. Transgenic pigs expressing near infrared fluorescent protein—A novel tool for noninvasive imaging of islet xenotransplants. Xenotransplantation, 21 December; doi.org/10.1111/xen.12719

• Reichart B, Längin M, Denner J, Schwinzer R, Cowan PJ, Wolf E. Pathways to Clinical Cardiac Xenotransplantation. Transplantation. Sep 1;105(9):1930-1943. doi: 10.1097/TP.0000000000003588.

2020

• Denner J, Längin M, Reichart B, Krüger L, Fiebig U, Mokelke M, Radan J, Mayr T, Milusev A, Luther F, Sorvillo N, Rieben R, Brenner P, Walz C, Wolf E, Roshani B, Stahl‑Hennig C, Abicht JM. Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival. Sci Rep Oct 16;10(1):17531. doi: 10.1038/s41598-020-73150-9.
  
  
• Reichart B, Längin M, Radan J, Mokelke M, Buttgereit I, Ying J, Fresch AK, Mayr T, Issl L, Buchholz S, Michel S, Ellgass R, Mihalj M, Egerer S, Baehr A, Kessler B, Kemter E, Kurome M, Zakhartchenko V, Steen S, Sjöberg T, Paskevicius A, Krüger L, Fiebig U, Denner J, Godehardt AW, Tönjes RR, Milusev A, Rieben R, Sfriso R, Walz C, Kirchner T, Ayares D, Lampe K, Schönmann U, Hagl C, Wolf E, Klymiuk N, Abicht JM, Brenner P. Pig-to-non-human primate heart transplantation: The final step toward clinical xenotransplantation? J Heart Lung Transplant. Aug;39(8):751-757.

2019

• Kola I, Egerer S, Bähr A, Godehardt AW, Tönjes RR, Ayares D, Wolf E, Klymiuk N. Removal of PERV- C proviruses from xenotransplanation donor pigs by marker-assisted breeding or gene editing. Xenotransplantation 2019;xen12553 [Abstract]
  
  
• Krüger L, Längin M, Reichart B, Fiebig U, Kristiansen Y, Prinz C, Kessler B, Egerer S, Wolf E, Abicht JM, Denner J. Transmission of Porcine Circovirus 3 (PCV3) by Xenotransplantation of Pig Hearts into Baboons. Viruses. Jul 16;11(7). pii: E650. doi: 10.3390/v11070650.

2018

• Abicht JM, Sfriso R, Reichart B, Längin M, Gahle K, Puga Yung GL, Seebach JD, Rieben R, Ayares D, Wolf E, Klymiuk N, Baehr A, Kind A, Mayr T, Bauer A. Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood. Xenotransplantation. Sep;25(5):e12390. doi: 10.1111/xen.12390. Epub 2018 Mar 14.
  
  
• Egerer S, Fiebig U, Kessler B, Zakhartchenko V, Kurome M, Reichart B, Kupatt C, Klymiuk N, Wolf E, Denner J, Bähr A. Early weaning completely eliminates porcine cytomegalovirus from a newly established pig donor facility for xenotransplantation. Xenotransplantation Jul. 25(4):e12449
  
  
• Fiebig U, Fischer K, Bähr A, Runge C, Schnieke A, Wolf E, Denner J. Porcine endogenous retroviruses: Quantification of the copy number in cell lines, pig breeds, and organs. Xenotransplantation Jul. 25(4):e12445.
  
  
• Fiebig U, Abicht JM, Mayr T, Längin M, Bähr A, Guethoff S, Falkenau A, Wolf E, Reichart B, Shibahara T, Denner J; Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation. Viruses, 10(2) Feb 6, E66; doi:10.3390/v10020066

• Hawthorne WJ, Cowan PJ, Bühler LH, Yi S, Bottino R, Pierson RN 3rd, Ahn C, Azimzadeh A, Cozzi E, Gianello P, Lakey JRT, Luo M, Miyagawa S, Mohiuddin MM, Park CG, Schuurman HJ, Scobie L, Sykes M, Tector J, Tönjes RR, Wolf E, Nuñez JR, Wang W. Third WHO Global Consultation on Regulatory Requirements for Xenotransplantation Clinical Trials, Changsha, Hunan, China December 12-14, 2018: "The 2018 Changsha Communiqué" The 10-Year Anniversary of The International Consultation on Xenotransplantation. Xenotransplantation. Mar;26(2):e12513. doi: 10.1111/xen.12513. Epub 2019 Apr 13. No abstract available.

• Hinrichs A, Kessler B, Kurome M, Blutke A, Kemter E, Bernau M, Scholz AM, Rathkolb B, Renner S, Bultmann S, Leonhardt H, de Angelis MH, Nagashima H, Hoeflich A, Blum WF, Bidlingmaier M, Wanke R, Dahlhoff M, Wolf E. Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver. Mol Metab. May;11:113-128. doi: 10.1016/j.molmet.2018.03.006. Epub 2018 Mar 15.
  
  
• Kemter E, Denner J, Wolf E. Will Genetic Engineering Carry Xenotransplantation of Pig Islets to the Clinic? Curr Diab Rep. Sep 18;18(11):103. doi: 10.1007/s11892-018-1074-5. Review.

• Kemter E, Wolf E. Recent progress in porcine islet isolation, culture and engraftment strategies for xenotransplantation. Curr Opin Organ Transplant. Dec;23(6):633-641. doi: 10.1097/MOT.0000000000000579.
  
  
• Kemter, E., Kind, A., Reichart, B. and Wolf, E. Use of Xenogeneic Cells. Cell Engineering and Regeneration (Springer International Publishing, Cham, 2018), vol. 61, pp. 1–46.

• Kleinert M, Clemmensen C, Hofmann SM, Moore MC, Renner S, Woods SC, Huypens P, Beckers J, de Angelis MH, Schürmann A, Bakhti M, Klingenspor M, Heiman M, Cherrington AD, Ristow M, Lickert H, Wolf E, Havel PJ, Müller TD, Tschöp MH. Animal models of obesity and diabetes mellitus. Nat Rev Endocrinol. Mar;14(3):140-162. doi: 10.1038/nrendo.2017.161. Epub 2018 Jan 19. Review.

• Kurome M, Baehr A, Simmet K, Jemiller EM, Egerer S, Dahlhoff M, Zakhartchenko V, Nagashima H, Klymiuk N, Kessler B, Wolf E. Targeting αGal epitopes for multi-species embryo immunosurgery. Reprod Fertil Dev. 2018 Nov 2. doi: 10.1071/RD18120.
  
  
• Längin M, Mayr T, Reichart B, Michel S, Buchholz S, Guethoff S, Dashkevich A, Baehr A, Egerer S, Bauer A, Mihalj M, Panelli A, Issl L, Ying J, Fresch AK, Buttgereit I, Mokelke M, Radan J, Werner F, Lutzmann I, Steen S, Sjöberg T, Paskevicius A, Qiuming L, Sfriso R, Rieben R, Dahlhoff M, Kessler B, Kemter E, Klett K, Hinkel R, Kupatt C, Falkenau A, Reu S, Ellgass R, Herzog R, Binder U, Wich G, Skerra A, Ayares D, Kind A, Schönmann U, Kaup FJ, Hagl C, Wolf E, Klymiuk N, Brenner P, Abicht J. Consistent success in life-supporting porcine cardiac xenotransplantation. Nature 5. Dez.;564, pages430–433
  
  
• Puga Yung G, Bongoni AK, Pradier A, Madelon N, Papaserafeim M, Sfriso R, Ayares DL, Wolf E, Klymiuk N, Bähr A, Constantinescu MA, Voegelin E, Kiermeir D, Jenni H, Rieben R, Seebach JD. Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood. Xenotransplantation. Jan;25(1). doi: 10.1111/xen.12357. Epub 2017 Oct 22.
  
  
• Rieblinger B, Fischer K, Kind A, Saller BS, Baars W, Schuster M, Wolf-van Buerck L, Schäffler A, Flisikowska T, Kurome M, Zakhartchenko V, Kessler B, Flisikowski K, Wolf E, Seissler J, Schwinzer R, Schnieke A. Strong xenoprotective function by single-copy transgenes placed sequentially at a permissive locus. Xenotransplantation Jan 23. doi: 10.1111/xen.12382.
  
  
• Wolf, E. et al., Genetisch modifizierte Schweine als Zell, Gewebe- und Organquelle. Tierische Organe in menschlichen Körpern (2018), pp. 65–86.

2017

• Buerck LW, Schuster M, Oduncu FS, Baehr A, Mayr T, Guethoff S, Abicht J, Reichart B, Klymiuk N, Wolf E, Seissler J. LEA29Y expression in transgenic neonatal porcine islet-like cluster promotes long-lasting xenograft survival in humanized mice without immunosuppressive therapy. Sci Rep. Jun 15;7(1):3572. doi: 10.1038/s41598-017-03913-4.
  
  
• Cohrs, C.M., Chen, C., Jahn, S.R., Stertmann, J., Chmelova, H., Weitz, J., Bähr, A., Klymiuk, N., Steffen, A., Ludwig, B., et al. Vessel Network Architecture of Adult Human Islets Promotes Distinct Cell-Cell Interactions In Situ and Is Altered After Transplantation. Endocrinology 158(5), 1373-1385.
  
  
• Hinkel R, Howe A, Renner S, Ng J, Lee S, Klett K, Kaczmarek V, Moretti A, Laugwitz KL, Skroblin P, Mayr M, Milting H, Dendorfer A, Reichart B, Wolf E, Kupatt C. Diabetes Mellitus-Induced Microvascular Destabilization in the Myocardium. J Am Coll Cardiol. Jan 17;69(2):131-143. doi: 10.1016/j.jacc.2016.10.058.
  
  
• Kemter E, Cohrs CM, Schäfer M, Schuster M, Steinmeyer K, Wolf-van Buerck L, Wolf A, Wuensch A, Kurome M, Kessler B, Zakhartchenko V, Loehn M, Ivashchenko Y, Seissler J, Schulte AM, Speier S, Wolf E. INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters. Diabetologia 60, 1152-1156.
  

2016

• Cooper DK, Matsumoto S, Abalovich A, Itoh T, Mourad NI, Gianello PR, Wolf E, Cozzi E. Progress in Clinical Encapsulated Islet Xenotransplantation.Transplantation Nov;100(11):2301-2308. Review.

• Bähr A, Käser T, Kemter E, Gerner W, Kurome M, Baars W, Herbach N, Witter K, Wünsch A, Talker SC, Kessler B, Nagashima H, Saalmüller A, Schwinzer R, Wolf E, Klymiuk N. Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs. PLoS One. 2016 May 13;11(5):e0155676. doi: 10.1371/journal.pone.0155676.
  
  
• Fischer K, Kraner-Scheiber S, Petersen B, Rieblinger B et al. Efficient production of multi-modified pigs for xenotransplantation by cobineering, gene stacking and gene editing. Nature Scientific Reports 2016; doi: 10.1038/srep29081
  
  
• Kourtzelis I, Kotlabova K, Lim JH, Mitroulis I, Ferreira A, Chen LS, Gercken B, Steffen A, Kemter E, Klotzsche-von Ameln A, Waskow C, Hosur K, Chatzigeorgiou A, Ludwig B, Wolf E, Hajishengallis G, Chavakis T. Developmental endothelial locus-1 modulates platelet-monocyte interactions and instant blood-mediated inflammatory reaction in islet transplantation. Thromb Haemost. Apr;115(4):781-8. doi: 10.1160/TH15-05-0429.
  
  
• Rataj D, Werwitzke S, Haarmeijer B, Winkler M, Ramackers W, Petersen B, Niemann H, Wünsch A, Bähr A, Klymiuk N, Wolf E, Abicht JM, Ayares D, Tiede A. Inhibition of complement component C5 prevents clotting in an ex vivo model of xenogeneic activation of coagulation. Xenotransplantation Mar;23(2):117-27. doi: 10.1111/xen.12218. Epub 2016 Jan 16. PubMed PMID: 26773447.

2015

• Kemter, E., and Wolf, E.  Pigs pave a way to de novo formation of functional human kidneys. Proceedings of the National Academy of Sciences of the United States of America 112, 12905-12906.
  
  

• Reichart B, Niemann H, Chavakis T, Denner J, Jaeckel E, Ludwig B, Marckmann G, Schnieke A, Schwinzer R, Seissler J, Tönjes RR, Klymiuk N, Wolf E, Bornstein SR. Xenotransplantation of porcine islet cells as a potential option for the treatment of type 1 diabetes in the future. Horm Metab Res; 47(1):31-5.

• Renner, S., Blutke, A., Streckel, E., Wanke, R., and Wolf, E. Incretin actions and consequences of incretin-based therapies – lessons from complementary animal models. Journal of Pathology Oct 12 (in press) (doi: 10.1002/path.4655).

• Wolf-van Buerck L, Schuster M, Baehr A, Mayr T, Guethoff S, Abicht J, Reichart B, Nam-Apostolopoulos Y, Klymiuk N, Wolf E, et al. Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine islet-like clusters. Xenotransplantation Oct 21., doi: 10.1111/xen.12201.

2014

• Graf, A., Krebs, S., Zakhartchenko, V., Schwalb, B., Blum, H., and Wolf, E. Fine mapping of genome activation in bovine embryos by RNA sequencing. Proceedings of the National Academy of Sciences of the United States of America 111, 4139-4144
  
  
• Klymiuk, N., Fezert, P., Wünsch, A., Kurome, M., Kessler, B., and Wolf, E. Homologous recombination contributes to the repair of zinc-finger-nuclease induced double strand breaks in pig primary cells and facilitates recombination with exogenous DNA. Journal of biotechnology 177, 74-81.

2013

• Renner, S., Braun-Reichhart, C., Blutke, A., Herbach, N., Emrich, D., Streckel, E., Wünsch, A., Kessler, B., Kurome, M., Bähr, A., Klymiuk, N., Krebs, S., Puk, O., Nagashima, H., Graw, J., Blum, H., Wanke, R., and Wolf, E. Permanent neonatal diabetes in INS(C94Y) transgenic pigs. Diabetes 62, 1505-1511.
  
  
• Lommel, M., Winterhalter, P.R., Willer, T., Dahlhoff, M., Schneider, M.R., Bartels, M.F., Renner-Muller, I., Ruppert, T., Wolf, E., and Strahl, S. Protein O-mannosylation is crucial for E-cadherin-mediated cell adhesion. Proceedings of the National Academy of Sciences of the United States of America 110, 21024-21029.
  
  
• Klymiuk, N., Blutke, A., Graf, A., Krause, S., Burkhardt, K., Wuensch, A., Krebs, S., Kessler, B., Zakhartchenko, V., Kurome, M., Kemter, E., Nagashima, H., Schoser, B., Herbach, N., Blum, H., Wanke, R., Aartsma-Rus, A., Thirion, C., Lochmuller, H., Walter, M.C., and Wolf, E. Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle. Human molecular genetics 22, 4368-4382.

2012

• Renner, S., Romisch-Margl, W., Prehn, C., Krebs, S., Adamski, J., Goke, B., Blum, H., Suhre, K., Roscher, A.A., and Wolf, E. Changing metabolic signatures of amino acids and lipids during the prediabetic period in a pig model with impaired incretin function and reduced beta-cell mass. Diabetes 61, 2166-2175.
  
  
• Klymiuk, N., van Buerck, L., Bähr, A., Offers, M., Kessler, B., Wuensch, A., Kurome, M., Thormann, M., Lochner, K., Nagashima, H., Herbach, N., Wanke, R., Seissler, J., and Wolf, E. Xenografted islet cell clusters from INSLEA29Y transgenic pigs rescue diabetes and prevent immune rejection in humanized mice. Diabetes 61, 1527-1532.
  
  
• Klymiuk, N., Mundhenk, L., Kraehe, K., Wuensch, A., Plog, S., Emrich, D., Langenmayer, M.C., Stehr, M., Holzinger, A., Kroner, C., Richter, A., Kessler, B., Kurome, M., Eddicks, M., Nagashima, H., Heinritzi, K., Gruber, A.D., and Wolf, E. Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis. Journal of molecular medicine 90, 597-608.

2011

• Klymiuk N, Mundhenk L, Kraehe K, Wuensch A, Plog S, Emrich D, Langenmayer MC, Stehr M, Holzinger A, Kröner C, Richter A, Kessler B, Kurome M, Eddicks M, Nagashima H, Heinritzi K, Gruber AD, Wolf E Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis. J Mol Med (Berl) Dec 15. [Epub ahead of print] PubMed PMID: 22170306.
  
  
• Klymiuk N, Böcker W, Schönitzer V, Bähr A, Radic T, Fröhlich T, Wünsch A, Keßler B, Kurome M, Schilling E, Herbach N, Wanke R, Nagashima H, Mutschler W, Arnold GJ, Schwinzer R, Schieker M, Wolf E. First inducible transgene expression in porcine large animal models. FASEB J Dec 12. [Epub ahead of print] PubMed PMID: 22138035.

2010

• Renner S, Fehlings C, Herbach N, Hofmann A, von Waldthausen DC, Kessler B, Ulrichs K, Chodnevskaja I, Moskalenko V, Amselgruber W, Göke B, Pfeifer A, Wanke R, Wolf E, Glucose intolerance and reduced proliferation of pancreatic beta-cells in transgenic pigs with impaired glucose-dependent insulinotropic polypeptide function. Diabetes 2010 May;59(5):1228-38. Epub Feb 25. PubMed PMID: 20185813.

 2009

• Bauersachs S, Ulbrich SE, Zakhartchenko V, Minten M, Reichenbach M, Reichenbach HD, Blum H, Spencer TE, Wolf E The endometrium responds differently to cloned versus fertilized embryos. Proc Natl Acad Sci U S A 2009 Apr 7;106(14):5681-6. Epub Mar 23. PubMed PMID: 19307558.
  
  
• Weiss EH, Lilienfeld BG, Müller S, Müller E, Herbach N, Kessler B, Wanke R, Schwinzer R, Seebach JD, Wolf E*, Brem G*. HLA-E/human beta2-microglobulin transgenic pigs: protection against xenogeneic human anti-pig natural killer cell cytotoxicity. Transplantation Jan 15;87(1):35-43. PubMed PMID: 19136889. *equal last-author contribution.