Ausgewählte Publikationen

A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of T-Cell Interactions with the Cervical Spinal Cord Microvasculature during Experimental Autoimmune Encephalomyelitis

Front Immunol. 2017 Apr 11;8:406. doi: 10.3389/fimmu.2017.00406. eCollection 2017.

Here we described how to make spinal cord window at cervical spinal cord to observe T cell migration. The results were compared one from lumber spinal cord. [mehr...]

Visualizing context-dependent calcium signaling in encephalitogenic T cells in vivo by two-photon microscopy

Proc Natl Acad Sci U S A. 2017 Jul 17. pii: 201701806. doi: 10.1073/pnas.1701806114. [Epub ahead of print]

Here we visualized intracellular calcium in the encephalitogenic T cells in vivo by using FRET based sensor. The T cells showed short and long lasting calcium signaling dependent on their interaction partners. The blocking of the calcium signaling can be used as target of therapeutic treatments. [mehr...]

Lymphocyte Circadian Clocks Control Lymph Node Trafficking and Adaptive Immune Responses

Immunity. 2017 Jan 6. pii: S1074-7613(16)30517-9.

Lymphocytes circulate through lymph nodes (LN) in search for antigen in what is believed to be a continuous process. Here, we show that lymphocyte migration through lymph nodes and lymph occurred in a non-continuous, circadian manner. [mehr...]

In vivo imaging in autoimmune diseases in the central nervous system

Allergol Int. 2016 Jul;65(3):235-42. doi: 10.1016/j.alit.2016.02.001. Epub 2016 Feb 28.

Intravital imaging is becoming more popular and is being used to visualize cellular motility and functions. [mehr...]

Post-CNS-inflammation expression of CXCL12 promotes the endogenous myelin/neuronal repair capacity following spontaneous recovery from multiple sclerosis-like disease

J Neuroinflammation. 2016 Jan 8;13(1):7. doi: 10.1186/s12974-015-0468-4.

These data link CXCL12 expression in the DG and CC of EAE-recovering mice to the promotion of neuro/oligodendrogenesis generating CXCR4+ CXCL12+ neuronal and oligodendrocyte progenitor cells endowed with intrinsic neuro/oligondendroglial differentiation potential. These findings highlight the post-CNS-inflammation role of CXCL12 in augmenting the endogenous myelin/neuronal repair capacity in MS-like disease, likely via CXCL12/CXCR4 autocrine signaling. [mehr...]

Treg cells mediate recovery from EAE by controlling effector T cell proliferation and motility in the CNS

Acta Neuropathol Commun. 2014 Dec 5;2(1):163.

Here, we showed the role of regulatory T cells in the CNS during inflammation. Two-photon imaging revealed the interaction with other T cells and antigen presenting cells. [mehr...]

From classic to spontaneous and humanized models of multiple sclerosis: impact on understanding pathogenesis and drug development

J Autoimmun. 2014 Nov;54:33-50. doi: 10.1016/j.jaut.2014.06.004. Epub 2014 Aug 28.

Reviewing the Experimental Autoimmune Encephalomyelitis as animal model for Multiple Sclerosis [mehr...]

T cell-activation in neuromyelitis optica lesions plays a role in their formation

Acta Neuropathol Commun. 2013 Dec 24;1(1):85. doi: 10.1186/2051-5960-1-85.

Here, we showed that T cell activation in the neuromyelitis optica lesions is critical step for disease onset. [mehr...]

Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator

Nature Medicine 2013 May 12; doi:10.1038/nm.3180 [Epub ahead of print]

Here, we introduce fluorescence resonance energy transfer (FRET) based activation sensor, which can be expressed stably in T cells. Intravital imaging detects the change of intracellular calcium level. [mehr...]

2-photon imaging of phagocyte-mediated T cell activation in the CNS

J Clin Invest. 2013;123(3):1192–1201. doi:10.1172/JCI67233

Here, we show T cell activation within the CNS by using NFAT-GFP fusion protein and two-photon intravital microscopy. A part of APCs in the spinal cord leptomeninges present endogenous antigens to infiltrated autoreactive T cells and activate them. [mehr...]

An autoimmunity odyssey: how autoreactive T cells infiltrate into the CNS

Immunol Rev. 2012 Jul;248(1):140-55

This manuscript summarizes fate of autoreactive T cells after adoptive transfer into the recipients. [mehr...]

Knocking at the brain's door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures

Semin Immunopathol. 2010 Sep;32(3):275-87. Epub 2010 Jul 11

Reviewing intravital imaging of autoreactive T cell infiltration into the CNS. [mehr...]

Nicotinic acid adenine dinucleotide phosphate-mediated calcium signalling in effector T cells regulates autoimmunity of the central nervous system

Brain. 2010 Jul;133(Pt 7):1930-43. Epub 2010 Jun 2

Effects of calcium channel inhibitor on animal model of autoimmunity. [mehr...]

Effector T cell interactions with meningeal vascular structures in nascent autoimmune CNS lesions

Nature. 2009 Nov 5;462(7269):94-8. Epub 2009 Oct 14

Intravital two-photon imaging first time shows how auto-antigen specific T cells infiltrate into the CNS. [mehr...]

Blood-borne soluble protein antigen intensifies T cell activation in autoimmune CNS lesions and exacerbates clinical disease

Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18625-30. Epub 2007 Nov 13

Soluble antigen treatment after onset of EAE induces disaster in the host. [mehr...]

Instant effect of soluble antigen on effector T cells in peripheral immune organs during immunotherapy of autoimmune encephalomyelitis

Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):920-5. Epub 2007 Jan 9

Soluble antigen treatment before onset of EAE ameliorates EAE dramatically. Intravital imaging shows T cell arrest in the spleen in short time after treatment. [mehr...]

Live imaging of effector cell trafficking and autoantigen recognition within the unfolding autoimmune encephalomyelitis lesion

J Exp Med. 2005 Jun 6;201(11):1805-14

Spinal cord acute slice imaging illustrates autoantigen specific T cells recognize endogenous antigen in the CNS parenchyma. [mehr...]


Prof. Dr.

Reinhard Hohlfeld


Prof. Dr.

Martin Kerschensteiner




Naoto Kawakami


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