Ontogeny of Megakaryocytes in Health and Disease


We are investigating the lineage-commitment of hematopoietic progenitor cells into megakaryo-erythroid precursors and finally mature megakaryocytes during mammalian development. Our aim is to track megakaryopoiesis in its different niches, revealing novel interactions with surrounding cells. Applying multiple imaging techniques, transgenic mouse models and flow cytometry sorting, we can dissect megakaryocyte development in a spatial and temporal manner in vivo. Our translational approach involves the functional analysis of in vitro derived megakaryocytes and platelets from patients with rare genetic diseases like Glanzmann thrombasthenia or MYH9-related disorders.  Therefore, we are differentiating patient-specific induced pluripotent stem cells into megakaryocytes to reveal pathophysiologic mechanisms on a cellular level and reconstruct early hematopoiesis in vitro.



PI Mathias Orban  mathias.orban@med.uni-muenchen.de



Key publications:


Functional Comparison of Induced Pluripotent Stem Cell- and Blood-Derived GPIIbIIIa Deficient Platelets.

Orban M, Goedel A, Haas J, Sandrock-Lang K, Gärtner F, Jung CB, Zieger B, Parrotta E, Kurnik K, Sinnecker D, Wanner G, Laugwitz KL, Massberg S, Moretti A.

PLoS One. 2015 Jan 21;10(1):e0115978. doi: 10.1371/journal.pone.0115978. eCollection 2015.