Jobs

The Institute of Clinical Neuroimmunology offers a

Master thesis in the Meinl Lab

Topic: Identification of brain-reactive antibodies in Multiple Sclerosis and related diseases

Background and question:
Multiple Sclerosis (MS) is the most common inflammatory demyelinating disease of the central nervous system (CNS) in young adults with a high risk of future disability. An important role of antibodies (Abs) and B cells has emerged only recently and is based on the beneficial therapeutic effects of B cell - targeted treatment in a subgroup of MS patients, the identification of B cells in meningeal lymphoid structures, and antibody and complement deposition in brain lesions of several patients. The ongoing search for novel antigens has been successful in diseases that can closely resemble MS . We aim to understand the prevalence of brain reactive Abs (BRAB) in different MS patients and related disease by establishing high flow throughput assays that also allow us to detect antibodies that require the antigen in a certain configuration. We aim to understand BRAB in MS patients and related diseases by establishing a high flow throughput assay. In addition we aim to identify a novel Antigen(AG) in patients who are positive for BRAB by using a combination of different assays, such as single cell sorting of B cells, analysis of BRAB-AG binding on a large protein array and other techniques. We will investigate the frequency of AG specific BRAB in MS patients, related diseases and controls. This approach might allow personalized treatment of patients, and maybe results in further subdivision of MS into separate disease entities.

Methods: Immunohistochemistry, Western blot, FACS, cell culture and others

Supervision: Dr. Simone Mader and Prof. Dr. Edgar Meinl

Prior experiences in laboratory work are required. Applications comprising of a curriculum vitae and relevant documents should be sent within a single PDF file via email to

Simone.Mader@med.uni-muenchen.de

Institut für Klinische Neuroimmunologie
Biomedizinisches Zentrum und
Klinikum der Ludwig-Maximilians-Universität München
Großhaderner Straße 9
82152 Martinsried


The Hospital of the University of Munich, Germany, is one of the largest and most competitive university hospitals in Germany and Europe. 47 specialized hospitals, departments and institutions harboring excellent research and education provide patient care at the highest medical level wit h approximately 10.000 employees.

Open position for a postdoctoral scientist (F/M)

T cell immunology, autoimmunity

Topic:

Antigens of Pathogenic T cells from Patients with
Ankylosing Spondylitis (Morbus Bechterew)

Background:
In collaboration with rheumatologists, we are investigating the pathogeneses of ankylosing spondylitis (AS). AS is a chronic inflammatory disease which primarily affects the axial skeleton. It is presumably an autoimmune disease and has a very strong genetic bias to HLA-B*27:05. Because HLA molecules present antigenic peptides to cytotoxic T cells, this bias suggests that arthritogenic peptides may play a key role in the pathogenesis of AS by initiating the autoimmune attack of the T cells against spinal tissue. However, arthritogenic peptides have not yet been identified. The current project aims at identifying such peptides in order to reveal new insights into the pathogenesis of AS. All required techniques are established in our lab.

In pilot experiments we have identified several expanded T cell clones in AS patients. Their T cell receptors share structural motifs which supports our hypothesis. So far, one receptor is already cloned and ready to be examined by a novel, highly sensitive technique for analyzing T cell antigens (K. Siewert et al Nat.Med. 2012; A. Arakawa et al. J.Exp.Med. 2015).

We are now planning to investigate the T cell receptor repertoires in greater detail by further Illumina-next-generation sequencing with a particular focus on single cell analysis. We will then express further particularly interesting receptors, use them for antigen searches, and for investigating T cell receptors and peptide antigens in tissue samples from AS patients.


We seek:
Applicants should hold a doctoral degree (preferentially a PhD). Most importantly, he/she should be highly motivated, interested in joining an  interdisciplinary team, and ideally have some experience molecular biology.


We offer:
The position is funded by a DFG grant for 2 years. We offer a TV-L E13 position and are planning to start early 2019. Our well-equipped labs are at the new Biomedical Center. We encourage applications of women. In case of equal qualification, disabled individuals will be preferentially considered. Presentation costs cannot be reimbursed.


Please apply to:
Klaus.Dornmair@med.uni-muenchen.de
Institute for Clinical Neuroimmunology
Biomedical Center and University Hospital of the LMU Munich
Großhaderner Str. 9, 82152 Martinsried, room N.B. 03.013
Tel. 089-2180-71664
http://ini.klinikum.uni-muenchen.de/dornmair_lab.


The laboratory of PD Dr. Naoto Kawakami at the Institute of Clinical Neuroimmunology seeks to fill the following position:

PhD Candidate (m/f)


The group employs experimental autoimmune encephalomyelitis (EAE) as an animal model of multiple sclerosis. Specifically, by using two-photon microscopy, we visualize autoantigen specific T cell motility throughout the entire process of T cell infiltration into the CNS in living animals (Bartholomäus et al., Nature 2009, Mues et al., Nature Medicine 2013, Pesic et al., J. Clin. Invest. 2013, Kyratsous et al., PNAS 2017).


For a new project part, we would like to further study the interactions of encephalitogenic T cells with the Blood-brain barrier and with glial cells as well as the resulting consequences by using intravital imaging together with advanced methods of molecular biology.


Candidates should hold a Master’s degree in Biology, Immunology or a related subject. We are looking for applicants who are highly motivated, good team players, and yet capable of working independently. We prefer candidates with basic experience in fluorescence/confocal microscopy, experimental animal handling, flow cytometry, and cell culture.

The salary will be in accordance with the German model for the public sector (TV-L E13, 65%). The University of Munich is an equal opportunity employer. Handicapped candidates with equal qualifications will be given preference. For more details about the group, please see http://www.klinikum.uni-muenchen.de/Institut-fuer-Klinische-Neuroimmunologie/en/forschung/ag_kawakami/ .


Please send your complete CV, certificates of university degrees and other relevant transcripts together with letters of recommendation to
PD Dr. Naoto Kawakami, PhD
Institute of Clinical Neuroimmunology,
Biomedical center, LMU Munich Grosshaderner Str 9,
82152 Martinsried Germany
naoto.kawakami@med.uni-muenchen.de


The AG Kümpfel is looking from 01.10.2018, initially limited to 04/2019, for

A student research assistant (m / w) up to 19 hours per week

Your area of ​​responsibility:
• Performing simple laboratory activities under guidance (human and murine samples)
• support clinical database entries

Our requirements:
• scientific background (eg as a supplement to biology studies)
• independent and very structured work
• reliability and accuracy
• knowledge of microsoft office

Our background: Our research investigates the role of NLRP3 inflammasome in the central nervous system in the development of autoimmune diseases and autoinflammatory syndromes. Our goal is to decipher the pathogenesis of sterile neuroinflammation and thus to identify new treatment strategies.

Your application - exclusively by e-mail - to:    
Dr. med.univ. Elisabeth Schuh, PhD
Elisabeth.schuh@med.uni-muenchen.de
Institute of Clinical Neuroimmunology
BioMedical Center - LMU
Ludwig-Maximilians-University
Grosshaderner Str. 9
82152 Planegg-Martinsried