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Coming up in "Methods in Molecular Biology"

23.05.2017 - Generation of in vitro model of Epithelial Mesenchymal Transition

The epithelial to mesenchymal transition, also called EMT, is a physiological process that occurs during embryonal development and homeostasis in adult life. Besides, EMT plays a role in cancer progression since it is a key process in invasion and metastasis, and therefore it constitutes a topic of great interest in cancer research.

Dr. Tarik Regad together with his team in John van Geest Cancer Research Centre at Nottingham Trent University generated an interesting in vitro model to study EMT involvement in prostate cancer progression using the lentiviral cell transduction method for DNA delivery. Moving from his model, Dr. Regad has identified a protein that is being investigated by the IMMUTRAIN ESR Anna Di Biase as a promising target in prostate cancer immunotherapy. A detailed step-by-step protocol of this work has been accepted by Methods in Molecular Biology and is going to be published as “Generation of in vitro model of Epithelial Mesenchymal Transition (EMT) via the expression of a cytoplasmic mutant form of PML”.